Recombinant spidroins of comparable size and mechanical function to native silk proteins have been successfully produced by engineered E. coli.
\nRecombinant spidroins have been expressed in transgenic plants, rice, and alfalfa. These expression platforms can potentially offer increased industrial-scale economic viability over alternative host systems.
\nCRISPR\/Cas9-based genome editing has been used to incorporate recombinant spidroin genes into the genome of B. mori. The resulting chimeric fibres spun by the silkworms displayed a tensile strength equivalent to native spider silk.
\nTransgenic sheep embryos containing a recombinant spidroin gene have been constructed using somatic cell nuclear transfer. The transgenic embryos contained a hair-follicle specific promoter for expressing recombinant spidroins within fibres of wool. However, transgenic offspring are yet to be obtained.<\/p>\n
Spider silk is renowned for its impressive mechanical properties. It is one of the strongest known biomaterials, possessing mechanical properties that outmatch both steel and Kevlar. However, the farming of spiders for their silk is unfeasible. Consequently, production of recombinant spider silk proteins (spidroins) in more amenable hosts is an exciting field of research. For large-scale production to be viable, a heterologous silk production system that is both highly efficient and cost effective is essential. Genes encoding recombinant spidroin have been expressed in bacterial, yeast, insect, and mammalian cells, in addition to many other platforms. This review discusses the recent advances in exploiting an increasingly diverse range of host platforms in the heterologous production of recombinant spidroins.<\/p>\n","protected":false},"excerpt":{"rendered":"
Recombinant spidroins of comparable size and mechanical function to native silk proteins have been successfully produced by engineered E. coli. Recombinant spidroins have been expressed in transgenic plants, rice, and alfalfa. These expression platforms can potentially offer increased industrial-scale economic viability over alternative host systems. CRISPR\/Cas9-based genome editing has been used to incorporate recombinant spidroin […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","nova_meta_subtitle":"","footnotes":""},"categories":[5572],"tags":[8793,12417,12402],"supplier":[11310],"class_list":["post-79866","post","type-post","status-publish","format-standard","hentry","category-bio-based","tag-biomaterials","tag-proteins","tag-silk","supplier-cell-magazine"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/79866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=79866"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/79866\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=79866"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=79866"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=79866"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=79866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}