Coculture systems can accommodate complex pathways that are difficult to engineer in a single cell. Mutualistic growth between the consortia members has been a successful strategy to maintain stable coculture systems.
\nProtein engineering has been used as a complementary approach to increase the pathway flux in case there is a limited catalytic activity of the wild type enzymes.
\nDe novo entry pathways circumvent limitations associated with the native isoprenoid pathways, facilitating the optimization of cell growth and isoprenoid production.
\nEnhanced formation of lipid bodies within the host leads to higher isoprenoid titers owing to increased storage capacity and solubility.
\nThe development of cell-free platforms is an emerging strategy offering manifold advantages, such as the precise control of reaction conditions and the absence of product toxicity.<\/p>\n
Metabolic engineering has emerged as an important tool for reconstructing heterologous isoprenoid metabolic pathways in microbial hosts. Here, we provide an overview of promising engineering strategies that have proven to be successful for the high-yield production of isoprenoids. Besides \u2018conventional\u2019 approaches, such as the \u2018push\u2013pull\u2019 and protein engineering to optimize the isoprenoid flux and limited catalytic activity of enzymes, we review emerging strategies in the field, including compartmentalization between synthetic consortia members, novel bypass pathways for isoprenoid synthesis, cell-free systems, and improvement of the lipid content to overcome storage isoprenoid limitations. Pitfalls, along with lessons learned from the application of these strategies, will be addressed with the hope of guiding future efforts toward cost-effective and sustainable production of isoprenoids.<\/p>\n","protected":false},"excerpt":{"rendered":"
Coculture systems can accommodate complex pathways that are difficult to engineer in a single cell. Mutualistic growth between the consortia members has been a successful strategy to maintain stable coculture systems. Protein engineering has been used as a complementary approach to increase the pathway flux in case there is a limited catalytic activity of the […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","nova_meta_subtitle":"","footnotes":""},"categories":[5572],"tags":[5840,17018,12417],"supplier":[11310,5112],"class_list":["post-74678","post","type-post","status-publish","format-standard","hentry","category-bio-based","tag-enzymes","tag-isoprenoid","tag-proteins","supplier-cell-magazine","supplier-elsevier"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/74678","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=74678"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/74678\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=74678"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=74678"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=74678"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=74678"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}