High-throughput screening (HTS) provides more possibilities for both rational and semirational engineering of microorganisms to obtain better industrial strains.
\nVarious mutagenesis and directed evolution approaches have been developed and applied to constructing more diverse mutant libraries and screening these libraries for more powerful industrial microorganisms.
\nIn addition to detection using UV\/visible and fluorescence spectroscopy, screening techniques based on electrochemical sensors and biosensors, as well as on Raman, IR, and near-IR spectroscopy, are being developed to fulfill the diverse requirements of HTS.
\nAdvances in automated systems make HTS less labor-intensive and smarter. Accomplishments in microfluidics and cell sorting have significantly accelerated screening speed and have decreased the cost of screening.<\/p>\n
Based on the development of automatic devices and rapid assay methods, various high-throughput screening (HTS) strategies have been established for improving the performance of industrial microorganisms. We discuss the most significant factors that can improve HTS efficiency, including the construction of screening libraries with high diversity and the use of new detection methods to expand the search range and highlight target compounds. We also summarize applications of HTS for enhancing the performance of industrial microorganisms. Current challenges and potential improvements to HTS in industrial biotechnology are discussed in the context of rapid developments in synthetic biology, nanotechnology, and artificial intelligence. Rational integration will be an important driving force for constructing more efficient industrial microorganisms with wider applications in biotechnology.<\/p>\n","protected":false},"excerpt":{"rendered":"
High-throughput screening (HTS) provides more possibilities for both rational and semirational engineering of microorganisms to obtain better industrial strains. Various mutagenesis and directed evolution approaches have been developed and applied to constructing more diverse mutant libraries and screening these libraries for more powerful industrial microorganisms. In addition to detection using UV\/visible and fluorescence spectroscopy, screening […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","nova_meta_subtitle":"","footnotes":""},"categories":[5572],"tags":[13825,5796,11615],"supplier":[11310],"class_list":["post-71279","post","type-post","status-publish","format-standard","hentry","category-bio-based","tag-biosensor","tag-biotechnology","tag-microorganisms","supplier-cell-magazine"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/71279","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=71279"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/71279\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=71279"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=71279"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=71279"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=71279"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}