{"id":53303,"date":"2015-02-12T07:22:59","date_gmt":"2015-02-12T06:22:59","guid":{"rendered":"https:\/\/renewable-carbon.eu\/news\/?p=53303"},"modified":"2018-06-01T12:10:30","modified_gmt":"2018-06-01T10:10:30","slug":"berkeley-research-greener-blue-jeans","status":"publish","type":"post","link":"https:\/\/renewable-carbon.eu\/news\/berkeley-research-greener-blue-jeans\/","title":{"rendered":"Berkeley Research: Greener Blue Jeans"},"content":{"rendered":"
\"John_Dueber_Zach_Russ_web\"
John Dueber (right) and bioengineering graduate student Zach Russ examine a culture of indigo-producing E. coli bacteria. Photo: Peg Skorpinski<\/figcaption><\/figure>\n

Who doesn\u2019t like blue jeans? They\u2019re practically wrinkle-proof. \u00a0The indigo dye that provides their distinctive color holds up to detergents, but ages into that soft, worn look. No wonder the average American wears jeans four days a week. No wonder it\u2019s a $66 billion a year industry, with three billion pairs of jeans manufactured each year.
\n<\/strong>
\nIndigo is one of the oldest dyes used for coloring textiles. For thousands of years it was extracted from tropical plants in Asia, the Middle East and the Americas. An indigo-dyed garment discovered in a Thebes excavation dates back to 2500 B.C.<\/p>\n

Commercial synthesis of indigo dye replaced the plant source around 1900. Today, the jean industry uses about 40,000 tons of indigo a year. But there is a dark side. \u00a0Industrial synthesis of indigo from petroleum is a \u201cdirty\u201d chemical process. Chemical production of indigo into an effective dye requires a chemical that becomes toxic to fish and some other aquatic life. And when sent to waste water treatment plants, it severely corrodes the piping.<\/p>\n

Jeans manufacturers are interested in finding a cleaner route to produce the iconic dye. Berkeley bioengineering professor John Dueber has studied the chemical steps plants use to naturally make indigo, and he thinks he has found an environmentally green way for the industry to churn out the dye without the use of the toxic compound.<\/p>\n

When plant leaves are healthy, a chemical precursor to indigo, called indican, is caged within a sugar molecule and isolated from the rest of the cell in an organelle. \u00a0Only when leaves are damaged is indican released from this compartment. The sugar protective cage is removed, allowing a chemical change that makes indigo. \u00a0Green leaves turn blue.<\/p>\n

Dueber\u2019s lab very recently identified the plant enzyme that is essential for adding the protective sugar cage. They plan to insert its gene into bacteria. Addition of a second gene as well as tweaks to a few of the bacteria\u2019s genes should enable the bacteria to produce indican.<\/p>\n

\"indican_dyed_bear500w\"
When indigo\u2019s chemical precursor is applied to cotton cloth, a single enzyme can free indigo to dye a white bear to blue. Photo: John Dueber<\/figcaption><\/figure>\n

Hundreds of gallons of the harmless bacteria growing in fermentation tanks would churn out indican, held within the sugar\u2019s molecular embrace. Later, outside the cell, a second enzyme could remove the protective glucose cage, triggering the final chemical transition to indigo. The result: environmentally cleaner jeans.<\/p>\n

\u201cTo find green solutions, our lab looks toward nature,\u201d Dueber says. \u201cWe thought going back to the plants would be smart. If we can identify the enzyme the plants \u00a0use to produce the sugar cage and clone \u00a0its gene, we think the microbes can make large quantities of indican for dyeing jeans without the use of highly \u2018dirty chemicals.\u2019\u201d<\/p>\n

The process would be cleaner in another way. Current indigo production starts with petroleum. But the microbes thrive on cheap and plentiful glucose.<\/p>\n

Grad students Tammy Hsu and Zachary Russ worked in a greenhouse last summer, planting seeds, harvesting about a pound of green leaves from a Japanese indigo plant and grinding them up. \u201cAs you grind the leaves,\u201d Dueber says, \u201cthey turn into a blue paste because you have released indican from its sugar cage.\u201d<\/p>\n

Dueber\u2019s project is supported by a five-year Bakar Fellowship. Since he is in the early stages of the novel indigo project, he needs the time the multi-year support provides to work out the science and then develop a viable commercial strategy.<\/p>\n

\u201cMoving our lab\u2019s research to market is something I have not been trained to do,\u201d he says. \u201cI\u2019m excited to be part of this fellowship that connects us with people who can help us identify exactly what we should be filing patents on, and how much proof-of-concept work should be done before we file.\u201d<\/p>\n

He\u2019s also finding that the practical business guidance is just what his students are looking for. \u201cThey want to move their research into the commercial world. For the next five years, they\u2019ll be able to learn from experts who know this world. It\u2019s a fantastic opportunity for all of us.\u201d<\/p>\n

The Berkeley indigo research started in 2013 as an entrant in an undergraduate competition called iGEM \u2014 the International Genetically Engineered Machines. Dueber \u00a0advised the Berkeley team. \u00a0Five undergrads and two graduate student mentors advanced the research, demonstrating that the planned chemical steps could be made to work efficiently and that indican can make a good dye. They received a gold medal and advanced to participate in the world competition.<\/p>\n

\u201cStudents want to make a difference with their research,\u201d Dueber says. \u201cWe\u2019re still going to focus our research on academically interesting questions, but when there are industrial applications, we want to be aware of that. We\u2019d all like to see our indigo research lead to greener blue jeans.\u201d<\/p>\n

 <\/p>\n

Additional Information<\/h3>\n

ABC News story – Berkeley Researchers to Make Blue Jeans Greener<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Who doesn\u2019t like blue jeans? They\u2019re practically wrinkle-proof. \u00a0The indigo dye that provides their distinctive color holds up to detergents, but ages into that soft, worn look. No wonder the average American wears jeans four days a week. No wonder it\u2019s a $66 billion a year industry, with three billion pairs of jeans manufactured each […]<\/p>\n","protected":false},"author":59,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","nova_meta_subtitle":"","footnotes":""},"categories":[5572],"tags":[14563,10408],"supplier":[22751],"class_list":["post-53303","post","type-post","status-publish","format-standard","hentry","category-bio-based","tag-color","tag-greenchemistry","supplier-university-of-california-berkeley-uc-berkeley"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/53303","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/59"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=53303"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/53303\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=53303"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=53303"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=53303"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=53303"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}