The new model system now makes it easier for them to investigate how starch is formed and what role is played by the enzymes involved. In future, it may be possible to use yeast to trial specific modifications of starch.<\/p>\n
Starch is only produced by plants and algae. Now, it can also be produced by yeast \u2013 or, at any rate, by the yeasts in the labs of the group led by Samuel Zeeman, Professor of Plant Biochemistry at ETH Zurich\u2019s Institute of Agricultural Sciences. The researchers have succeeded in implanting yeast with the machinery that plants use to create this stored form of glucose. \u201cIt\u2019s an unprecedented innovation,\u201d says Zeeman.<\/p>\n
Transferring plant enzymes
\nTo achieve this aim, Barbara Pfister, the researcher spearheading the project, took the blueprints for seven different enzymes involved in starch synthesis from the genome of thale cress (Arabidopsis thaliana). They then implanted these into the yeast\u2019s genome, from which they also removed all enzymes involved in the synthesis of glycogen, the storage form of glucose in yeast, to prevent these enzymes from interfering with the synthesis of starch.<\/p>\n
In total, the researchers generated over 200 strains of yeast, some of them with all seven enzymes and others with various reduced sets of them. Accordingly, the strains produced either starch that closely resembled that of Arabidopsis or no starch at all \u2013 or starch products with varying degrees of modification.<\/p>\n
Yeast actually produces starch<\/p>\n
The researchers used various methods to investigate what exactly was produced by the yeast. In addition to the classical iodine test and various well-established imaging processes, the new technique of cryo X-ray ptychographic tomography developed at the Paul Scherrer Institute was applied here to determine the mass density of the synthesized products inside the yeast.<\/p>\n
The result: strains containing all seven enzymes produced starch with only minimal differences from Arabidopsis starch. However, what was surprising were the products of strains in which one or more enzymes were missing: depending on the combination, some of these strains nevertheless produced some type of starch.<\/p>\n
Starch by a roundabout route
\n\u201cStarch synthesis is not a linear process,\u201d explains Zeeman. \u201cIf an enzyme is missing, the ones that are left keep working anyway and just build a slightly different product.\u201d The researchers were able to show that, depending on the combination of the other enzymes, starch synthesis also works without debranching enzymes. These enzymes remove excess branching in the sugar chains produced during starch synthesis and were previously proposed to be indispensable to starch formation.<\/p>\n
\u201cAt present, the yeast system is purely a research tool,\u201d says the ETH professor. He explains that it allows starch synthesis to be simulated and influenced, as well as allowing more detailed investigation of the individual roles of participating enzymes and of the formation of starch\u2019s complicated structure. \u201cDoing these studies in yeast is far faster and simpler than in plants,\u201d emphasises Zeeman. Asked about future applications, he adds: \u201cOf course, it would be possible to try out novel starch modifications in the yeast system to attempt to improve starch properties for certain areas of application.\u201d<\/p>\n
Taking a new direction with yeast
\nThis SNF-funded project saw Zeeman\u2019s group work with yeast for the first time. \u201cIt worked remarkably well\u201d, he says. Zeeman and Pfister now want to use the yeast system to investigate starch synthesis in greater detail at the system level. To this end, the group has started working with mathematical modelling specialists in order to simulate the process in a computer model.<\/p>\n
Starch is an important constituent of foodstuffs such as maize, rice or potatoes. It is of major interest for the manufacture of biodegradable materials, and finds use in many unexpected places, such as in coating for paper. As such, starch is being constantly optimised for its various applications.<\/p>\n","protected":false},"excerpt":{"rendered":"
The new model system now makes it easier for them to investigate how starch is formed and what role is played by the enzymes involved. In future, it may be possible to use yeast to trial specific modifications of starch. Starch is only produced by plants and algae. Now, it can also be produced by […]<\/p>\n","protected":false},"author":74,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","nova_meta_subtitle":"","footnotes":""},"categories":[5572],"tags":[5840,5817],"supplier":[277,959,3507],"class_list":["post-39043","post","type-post","status-publish","format-standard","hentry","category-bio-based","tag-enzymes","tag-research","supplier-eidgenoessische-technische-hochschule-zuerich-eth-zuerich","supplier-paul-scherrer-institut-psi","supplier-schweizerischer-nationalfonds-snf"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/39043","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/74"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=39043"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/39043\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=39043"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=39043"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=39043"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=39043"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}