{"id":21207,"date":"2014-06-30T03:15:22","date_gmt":"2014-06-30T01:15:22","guid":{"rendered":"https:\/\/renewable-carbon.eu\/news\/?p=21207"},"modified":"2014-06-28T11:04:35","modified_gmt":"2014-06-28T09:04:35","slug":"jbei-gt-collection-new-resource-advanced-biofuels-research","status":"publish","type":"post","link":"https:\/\/renewable-carbon.eu\/news\/jbei-gt-collection-new-resource-advanced-biofuels-research\/","title":{"rendered":"The JBEI GT Collection: A New Resource for Advanced Biofuels Research"},"content":{"rendered":"
\"Josh-rice-plant-300x199\"
The JBEI GT Collection, the first glycosyltransferase clone collection specifically targeted for the study of plant cell wall biosynthesis, features GT clones of rice (shown here) and Arabidopsis plants. (Photo by Roy Kaltschmidt)<\/figcaption><\/figure>\n

Researchers at the U.S. Department of Energy (DOE)\u2019s Joint BioEnergy Institute (JBEI) have unveiled the first glycosyltransferase clone collection specifically targeted for the study of the biosynthesis of plant cell walls. The idea behind what is being called \u201cthe JBEI GT Collection\u201d is to provide a functional genomic resource for researchers seeking to extract the sugars in plant biomass and synthesize them into clean, green and renewable transportation fuels.<\/strong><\/p>\n

To address this problem, especially as it pertains to cell wall biosynthesis, a large team of JBEI researchers, led by Joshua Heazlewood, director of JBEI\u2019s Plant Systems Biology program, has cloned and verified a clone library consisting of 403 Arabidopsis GTs and 96 rice GTs. In plant biology, Arabidopsis is the reference plant for species like poplar, and rice the reference plant for grasses.<\/p>\n

\u201cUsing the unique infrastructure and resources at JBEI, we have provided a collection of high quality GT clones, all of which have been verified by sequencing and are available in easy to use cassettes,\u201d Heazelwood says. \u201cWe\u2019re making this entire collection available to the plant research community and expect it to drive our basic understanding of GTs and enable the manipulation of cell walls.\u201d<\/p>\n

In addition to the clones for Arabidopsis and rice GTs, Heazlewood and his collaborators at JBEI also created a set of highly efficient particle bombardment plasmids \u2013 pBullets \u2013 which are plasmids shot into a cell to mark the location of targeted proteins. The JBEI pBullets are constructed with markers for the plant endomembrane system, the collection of membranes that separates a cell\u2019s functional and structural compartments.<\/p>\n

\u201cOur pBullet vector series is custom designed for efficient bombardment,\u201d Heazlewood says. \u201cResearchers generally use large unwieldy plasmids that perform badly when it comes to localizing proteins.\u201d<\/p>\n

While the 403 Arabidopsis clones represent approximately 88-percent of the defined Arabidopsis GTs, the 96 rice clones represent only 15-percent of the defined rice GTs. JBEI researchers are now working to expand this. Both the JBEI GT Collection and pBullet vector series are available to the research community through various outlets. For more information visit the Website at http:\/\/gt.jbei.org\/<\/a><\/p>\n

Heazlewood and his collaborators have published a paper on the JBEI GT Collection in The Plant Journal. The paper is titled \u201cThe Plant Glycosyltransferase Clone Collection for Functional Genomics.\u201d Co-authors were Jeemeng Lao, Ai Oikawa, Jennifer Bromley, Peter McInerney, Anongpat Suttangkakul, Andreia Smith-Moritz, Hector Plahar, Tsan-Yu Chiu, Susana Gonz\u00e1lez Fern\u00e1ndez-Ni\u00f1o, Berit Ebert, Fan Yang, Katy Christiansen, Sara Hansen, Solomon Stonebloom, Paul Adams, Pamela Ronald, Nathan Hillson, Masood Hadi, Miguel Vega-S\u00e1nchez, Dominique Loqu\u00e9 and Henrik Scheller.<\/p>\n

This research was funded by the U.S. Department of Energy\u2019s Office of Science.<\/p>\n","protected":false},"excerpt":{"rendered":"

Researchers at the U.S. Department of Energy (DOE)\u2019s Joint BioEnergy Institute (JBEI) have unveiled the first glycosyltransferase clone collection specifically targeted for the study of the biosynthesis of plant cell walls. The idea behind what is being called \u201cthe JBEI GT Collection\u201d is to provide a functional genomic resource for researchers seeking to extract the […]<\/p>\n","protected":false},"author":59,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","nova_meta_subtitle":"","footnotes":""},"categories":[5572],"tags":[],"supplier":[7448,2869,2440,7447,4116],"class_list":["post-21207","post","type-post","status-publish","format-standard","hentry","category-bio-based","supplier-jbeigt","supplier-joint-bioenergy-institute-jbei","supplier-lawrence-berkeley-national-laboratory","supplier-the-plant","supplier-us-doe-office-of-science-sc"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/21207","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/59"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=21207"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/21207\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=21207"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=21207"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=21207"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=21207"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}