{"id":170049,"date":"2025-11-10T07:20:00","date_gmt":"2025-11-10T06:20:00","guid":{"rendered":"https:\/\/renewable-carbon.eu\/news\/?p=170049"},"modified":"2025-11-10T12:38:10","modified_gmt":"2025-11-10T11:38:10","slug":"neural-network-finds-an-enzyme-that-can-break-down-polyurethane","status":"publish","type":"post","link":"https:\/\/renewable-carbon.eu\/news\/neural-network-finds-an-enzyme-that-can-break-down-polyurethane\/","title":{"rendered":"Neural network finds an enzyme that can break down polyurethane"},"content":{"rendered":"\n\n\n

You\u2019ll often hear plastic pollution referred to as a problem. But the reality is that it\u2019s multiple problems. Depending on the properties we need, we form plastics out of different polymers, each of which is held together by a distinct type of chemical bond. So the method we use to break down one type of polymer may be incompatible with the chemistry of another.<\/p>\n\n\n

\n
\"The
The new $150.00 men”s “Nike Shox” basketball shoe, a hard to find and potential hot holiday item, is displayed at a Finish Line sporting goods store November 28, 2000 at Woodfield Shopping Mall in Schaumburg, IL. After trying steel springs and various high-tech designs to improve the cushioning of athletic shoes, Nike says they have finally found a formula that offers a comfortable bounce and is durable. The secret to the spring is the four columns of polyurethane foam originally used to absorb shock in Formula One race cars. The shoe cushions the foot from the impact of running and seems to channel that energy into your next step, Nike says.
\u00a9 Tim Boyle\/Newsmakers<\/figcaption><\/figure><\/div>\n\n\n

That problem is why, even though we\u2019ve had success finding enzymes that break down common plastics like\u00a0polyesters<\/a> and\u00a0PET<\/a>, they\u2019re only partial solutions to plastic waste. However, researchers aren\u2019t sitting back and basking in the triumph of partial solutions, and they\u2019ve now got very sophisticated protein design tools to help them out.<\/p>\n\n\n\n

That\u2019s the story behind a completely new enzyme that researchers developed to break down polyurethane, the polymer commonly used to make foam cushioning, among other things. The new enzyme is compatible with an industrial-style recycling process that breaks the polymer down into its basic building blocks, which can be used to form fresh polyurethane.<\/p>\n\n\n\n

Breaking down polyurethane<\/h3>\n\n\n\n
<\/div>\n\n\n
\n
\"Image
The basics of the chemical bonds that link polyurethanes. The rest of the polymer is represented by X\u2019s here.<\/figcaption><\/figure><\/div>\n\n\n

The new paper <\/a>that describes the development of this enzyme lays out the scale of the problem: In 2024, we made 22 million metric tons of polyurethane. The urethane bond that defines these involves a nitrogen bonded to a carbon that in turn is bonded to two oxygens, one of which links into the rest of the polymer. The rest of the polymer, linked by these bonds, can be fairly complex and often contains ringed structures related to benzene.<\/p>\n\n\n\n

Digesting polyurethanes is challenging. Individual polymer chains are often extensively cross-linked, and the bulky structures can make it difficult for enzymes to get at the bonds they can digest. A chemical called diethylene glycol can partially break these molecules down, but only at elevated temperatures. And it leaves behind a complicated mess of chemicals that can\u2019t be fed back into any useful reactions. Instead, it\u2019s typically incinerated as hazardous waste.<\/p>\n\n\n\n

To find something that could work better, the research team focused on finding an enzyme that could be integrated into the process with diethylene glycol. To begin, they tested all the enzymes reported in the literature as capable of breaking down polyurethanes. After testing all 15 of them, only three had decent activity against the polymer they were testing with, and they largely failed to break the polymer down to its constituent starting materials.<\/p>\n\n\n\n

So, the researchers focused on the enzyme that had the highest activity, searching for related proteins in public databases, and using the AlphaFold database of predicted structures to identify more distantly related proteins that folded up into a similar structure. On their own, none of these worked especially well either. But they turned out to be useful because they could be used to train an AI to look for sequences that could fold up into a similar structure.<\/p>\n\n\n\n

A new enzyme<\/h3>\n\n\n\n

The tool the team started working with is called Pythia-Pocket<\/a>, which is a neural network that specializes in determining whether any given amino acid in a protein is likely to contact whatever chemicals that structure can bind, along with any other functional features. That was combined with plain old Pythia (also a neural network), which predicts whether any given protein is likely to form a stable structure.<\/p>\n\n\n\n

The researchers reasoned that a good candidate for breaking down polyurethane would have a number of features. It would look, structurally, like the enzyme they had already been working with. It would also face a trade-off between having a structure that was ordered enough to form a similar binding pocket that would have enzymatic activity, but not so rigid that it couldn\u2019t flexibly fit around different types of polyurethanes. To strike this balance, the team used a message-passing interface that updated amino acid positions with each pass and balanced optimizing the structure and binding pocket. They called the resulting software GRASE, for graph neural network-based recommendation of active and stable enzymes.<\/p>\n\n\n\n

The results were pretty spectacular. Of the 24 most highly rated proteins the software evaluated, 21 of them showed some catalytic activity, and eight did better than the best enzyme we had known about previously. The best of these designs had 30 times the activity of that enzyme.<\/p>\n\n\n\n

Things got even better when the researchers mixed in the diethylene glycol and heated the mixture up to 50\u00b0 C. Under those conditions, the newly designed enzyme was over 450 times as active as the best-performing natural enzyme. It took 12 hours, but it could break down 98 percent of the polyurethane in the reaction mixture. And the enzyme was stable enough that it could be given a fresh mixture of polyurethane two additional times before its enzymatic activity started to wear out.<\/p>\n\n\n\n

Shifting from lab tests to kilogram-scale digestion showed the same thing: 95 percent or more of the material was broken down into the starting materials the polyurethane was made from.<\/p>\n\n\n\n

The researchers highlight the fact that their tools go beyond simply focusing on the structure formed by the protein, but incorporate information about its function, such as its stability and the amino acids that are likely to interact with the material it\u2019s digesting. And they suggest that these approaches may tell us more about how to get functional proteins by focusing on forming a similar 3D structure.<\/p>\n\n\n\n

Reference<\/h3>\n\n\n\n

Science, 2025. DOI: 10.1126\/science.adw4487<\/a> (About DOIs<\/a>).<\/p>\n","protected":false},"excerpt":{"rendered":"

You\u2019ll often hear plastic pollution referred to as a problem. But the reality is that it\u2019s multiple problems. Depending on the properties we need, we form plastics out of different polymers, each of which is held together by a distinct type of chemical bond. So the method we use to break down one type of […]<\/p>\n","protected":false},"author":59,"featured_media":170052,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"none","nova_meta_subtitle":"Given a dozen hours, the enzyme can turn a foam pad into reusable chemicals","footnotes":""},"categories":[17143],"tags":[5840,19036,17011,10453],"supplier":[7471],"class_list":["post-170049","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-recycling","tag-enzymes","tag-plasticfoam","tag-polyurethanes","tag-recycling","supplier-chinese-academy-sciences"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/170049","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/59"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=170049"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/170049\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media\/170052"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=170049"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=170049"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=170049"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=170049"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}