This forum focuses on research targets for promoting biofilm engineering to maximize the beneficial features of biofilms and to effectively utilize them in biofilm-mediated fermentation.<\/p>\n\n\n\n
DOI:https:\/\/doi.org\/10.1016\/j.tibtech.2023.11.012<\/a><\/p>\n\n\n\n The biofilm matrix can be thought of as a shared space for encased microbial cells; it consists of a wide variety of extracellular polymeric substances (EPSs) such as polysaccharides, proteins, amyloids, lipids, and extracellular DNA (eDNA). Most microbial cells can inherently form biofilms, which are more protective than planktonic cells against harsh conditions. Consequently, biofilm-based catalysis is characterized by high cell intensity and high tolerance in the fermentation process [1].<\/p>\n\n\n\n Although biofilm formation is an intrinsic ability of some microorganisms, not all microbial cells can form effective biofilms. Therefore, chassis cells must first be able to produce effective EPSs to form biofilms during fermentation. Currently, proteins present in the EPS matrix frequently serve as targets to engineer effective biofilms. A prominent example is the Escherichia coli<\/em> CsgA-based biofilm. CsgA is a secreted protein monomer that can be overexpressed to self-assemble into curli<\/p>\n\n\n\n Practical applications of biofilms should also avoid the continuous production of EPSs, which may create extremely dense biofilms and block the transmission of energy and oxygen during biofilm-mediated fermentation. Thus, engineering controllable biofilms is more important in fermentations. A simple strategy is to use inducible promoters rather than constitutive promoters to regulate the expression of biofilm components (such as CsgA and Psl) [11]. This strategy can regulate biofilm intensity<\/p>\n\n\n\n The synthesis of biofilm components, such as exopolysaccharides, is part of the cellular competition for a common carbon source, thereby reducing the yield of desired products during fermentation. For example, Psl is a repeating pentasaccharide containing D-mannose, D-glucose, and L-rhamnose; the synthesis of these monosaccharides will compete with the common sugar precursors, specifically glucose-1-phosphate (G-1-P) [13]. This process requires a significant amount of energy for biofilm<\/p>\n\n\n\n Effective control of biofilm structure and dynamics could facilitate numerous biotechnological applications. Biofilm fermentations have the potential to enhance productivity and product yields by promoting biomass retention and enabling continuous operation at high dilution rates. Furthermore, biofilms exhibit tolerance towards hazardous environments due to the protecting properties of the biofilm EPSs. This, in turn, enhances the conversion of crude biomass under substrate and product<\/p>\n\n\n\n The authors thank Dr Luyan Z. Ma at the Institute of Microbiology, Chinese Academy of Sciences, for constructive suggestions for the manuscript. This work was supported by the\u00a0National Key R & D Program of China(2023YFC3905000) and the\u00a0National Natural Science Foundation of China(32100018,\u00a021978129).<\/a><\/p>\n","protected":false},"excerpt":{"rendered":" Abstract This forum focuses on research targets for promoting biofilm engineering to maximize the beneficial features of biofilms and to effectively utilize them in biofilm-mediated fermentation. DOI:https:\/\/doi.org\/10.1016\/j.tibtech.2023.11.012 Section snippets The emergence of biofilm-based catalysis The biofilm matrix can be thought of as a shared space for encased microbial cells; it consists of a wide variety […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"none","nova_meta_subtitle":"Biofilm-based fermentation has great potential, as it possesses inherent characteristics such as self-immobilization, high resistance to reactants, and long-term activity","footnotes":""},"categories":[5572],"tags":[16380,13230,21623,16171],"supplier":[7471,23336,8801],"class_list":["post-137121","post","type-post","status-publish","format-standard","hentry","category-bio-based","tag-biobased","tag-biofilms","tag-eps","tag-fermentation","supplier-chinese-academy-sciences","supplier-national-key-rd-program-of-china","supplier-nnsf-china"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/137121","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=137121"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/137121\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=137121"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=137121"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=137121"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=137121"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Section snippets<\/h3>\n\n\n\n
The emergence of biofilm-based catalysis<\/h3>\n\n\n\n
Biofilm engineering using exopolysaccharides<\/h3>\n\n\n\n
Engineering controllable biofilms<\/h3>\n\n\n\n
Reuse of biofilm components<\/h3>\n\n\n\n
Concluding remarks<\/h3>\n\n\n\n
Acknowledgments<\/h3>\n\n\n\n