{"id":136053,"date":"2023-12-08T07:11:00","date_gmt":"2023-12-08T06:11:00","guid":{"rendered":"https:\/\/renewable-carbon.eu\/news\/?p=136053"},"modified":"2023-12-06T10:08:03","modified_gmt":"2023-12-06T09:08:03","slug":"releasing-brakes-on-biocatalysis","status":"publish","type":"post","link":"https:\/\/renewable-carbon.eu\/news\/releasing-brakes-on-biocatalysis\/","title":{"rendered":"Releasing Brakes on Biocatalysis"},"content":{"rendered":"\n\n
\n
\"Anja
Anja Hemschemeier, Jifu Duan and Eckhard Hofmann (from left) worked together on the study.\u00a0\u00a9\u00a0RUB, Marquard<\/figcaption><\/figure><\/div>\n\n\n

Enzymes from microorganisms can produce hydrogen (H2<\/sub>) under certain conditions, which makes them potential biocatalysts for biobased H2<\/sub>\u00a0technologies. In order to make this hydrogen production efficient, researchers are trying to identify and eliminate possible limiting factors. These include formaldehyde, which occurs naturally as a metabolic product in cells and inhibits the particularly efficient [FeFe] hydrogenase. A team from the work group Photobiotechnology at Ruhr University Bochum, Germany, was able to elucidate and switch off the underlying mechanism. The researchers report in the\u00a0Journal of the American Chemical Society from 20. November 2023<\/a>.<\/strong><\/p>\n\n\n\n

How a preservative affects H2<\/sub>-forming biocatalysts<\/h3>\n\n\n\n

Formaldehyde is known as a preservative, among other things, but it also occurs as a natural metabolite in living cells. Twelve years ago, scientists from the University of Oxford, UK, and Ruhr University Bochum, Germany, showed that this omnipresent molecule inhibits a certain class of biocatalysts, namely the particularly efficient hydrogen-generating hydrogenases of the two-iron type \u2013 so-called [FeFe]-hydrogenases. <\/p>\n\n\n\n

\n

\u201cThis was an interesting discovery, because formaldehyde could inhibit both the natural H2<\/sub>metabolism of microorganisms and isolated hydrogenases in biotechnological applications,\u201d explains Dr Jifu Duan, first author of the study.<\/p>\n<\/blockquote>\n\n\n\n

Molecular mechanism of formaldehyde poisoning elucidated<\/h3>\n\n\n\n

After various theoretical studies had hypothesized how the formaldehyde molecule might influence [FeFe]-hydrogenases, a team of researchers led by Jifu Duan and Professor Eckhard Hofmann at Ruhr University has now succeeded in elucidating the molecular mechanism experimentally. Using structures of formaldehyde-treated [FeFe]-hydrogenases obtained by protein crystallography, they were able to show that formaldehyde reacts with the so-called active center of the biocatalysts \u2013 an inorganic protein part where protons and electrons are converted to H2<\/sub>. In addition, however, formaldehyde combines with another very important protein part, which is necessary for the transport of protons to the active center by means of a sulfur-containing chemical group. When the scientists replaced this part with another, formaldehyde was hardly able to exert its inhibitory effect.<\/p>\n\n\n\n

The new findings could play a role in H2<\/sub> technologies<\/h3>\n\n\n\n
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\u201cFuture biotechnological applications of [FeFe]-hydrogenases may well involve the presence of formaldehyde, so that our modified formaldehyde-resistant biocatalysts could be used here,\u201d explains Jifu Duan. \u201cWe also believe that our findings can be transferred to other biocatalysts.\u201d <\/p>\n<\/blockquote>\n\n\n\n

This could play a role for bio-based industrial processes, but also for understanding metabolic pathways in living organisms.<\/p>\n\n\n\n

Funding<\/h3>\n\n\n\n

The researchers received financial support from German Research Foundation (Deutsche Forschungsgemeinschaft: individual grant and MiCon research training group).<\/p>\n\n\n\n

Original publication<\/h3>\n\n\n\n

Jifu Duan, Astrit Veliju, Oliver Lampret, Lingling Liu, Yadav Shanika, Ulf-Peter Apfel, Fraser Armstrong, Anja Hemschemeier, Eckhard Hofmann: Insights Into the Molecular Mechanism of Formaldehyde Inhibition of [FeFe]-hydrogenases, Journal of the American Chemical Society, 2023, DOI: 10.1021\/jacs.3c07800<\/a><\/p>\n\n\n\n

Press contact<\/h3>\n\n\n\n

Dr. Jifu Duan
Photobiotechnology Group
Department of Plant Biochemistry
Faculty for Biology and Biotechnology
Ruhr University Bochum
Germany
Phone: +49 234 32 24496
Email: jifu.duan@ruhr-uni-bochum.de<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Enzymes from microorganisms can produce hydrogen (H2) under certain conditions, which makes them potential biocatalysts for biobased H2\u00a0technologies. In order to make this hydrogen production efficient, researchers are trying to identify and eliminate possible limiting factors. These include formaldehyde, which occurs naturally as a metabolic product in cells and inhibits the particularly efficient [FeFe] hydrogenase. […]<\/p>\n","protected":false},"author":3,"featured_media":136056,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"none","nova_meta_subtitle":"Formaldehyde can inhibit enzymes that produce hydrogen particularly efficiently. Researchers from Bochum have discovered how this can be prevented","footnotes":""},"categories":[5572],"tags":[11833,5796,10630,23178],"supplier":[1143,1544,1806,2802],"class_list":["post-136053","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bio-based","tag-biocatalysis","tag-biotechnology","tag-hydrogen","tag-metabolite","supplier-american-chemical-society-acs","supplier-deutsche-forschungsgemeinschaft-dfg","supplier-ruhr-universitaet-bochum","supplier-university-of-oxford"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/136053","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=136053"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/136053\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media\/136056"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=136053"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=136053"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=136053"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=136053"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}