{"id":128128,"date":"2023-06-15T07:12:00","date_gmt":"2023-06-15T05:12:00","guid":{"rendered":"https:\/\/renewable-carbon.eu\/news\/?p=128128"},"modified":"2023-06-13T09:22:00","modified_gmt":"2023-06-13T07:22:00","slug":"milligrams-to-kilograms-making-microbes-work-at-scale","status":"publish","type":"post","link":"https:\/\/renewable-carbon.eu\/news\/milligrams-to-kilograms-making-microbes-work-at-scale\/","title":{"rendered":"Milligrams to kilograms: making microbes work at scale"},"content":{"rendered":"\n\n\n<ul class=\"wp-block-list\" id=\"l0005\"><li>Industrial cultivations suffer limitations in mixing and chemical feedstocks that cannot always match the laboratory-scale equivalent. As a result, normal laboratory-scale experiments do not capture relevant biological stresses, leading to underperformance at scale. <\/li><li>Bacterial stress response can be studied from the point of view of industrial-scale stresses to better inform strain design.<\/li><li>Strain design for industrial conditions can include removing unnecessary genes to improve performance under stressed and unstressed conditions.<\/li><li>Minimizing cellular adaptation may be a key to improving bioprocess production at scale.<\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"secst0010\">Abstract<\/h3>\n\n\n\n<p>If biomanufacturing can become a sustainable route for producing chemicals, it will provide a critical step in reducing greenhouse gas emissions to fight climate change. However, efforts to industrialize microbial synthesis of chemicals have met with varied success, due, in part, to challenges in translating laboratory successes to industrial scale. With a particular focus on&nbsp;<em>Escherichia coli<\/em>, this review examines the lessons learned when studying microbial physiology and metabolism under conditions that simulate large-scale bioreactors and methods to minimize cellular waste through reduction of maintenance energy, optimizing the stress response and minimizing culture heterogeneity. With general strategies to overcome these challenges, biomanufacturing process scale-up could be de-risked and the time and cost of bringing promising syntheses to market could be reduced.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"secst0015\">Keywords<\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li><a href=\"https:\/\/www.cell.com\/action\/doSearch?AllField=%22scale%20down%22&amp;ISSN=0167-7799\">scale down<\/a><\/li><li><a href=\"https:\/\/www.cell.com\/action\/doSearch?AllField=%22genome%20minimization%22&amp;ISSN=0167-7799\">genome minimization<\/a><\/li><li><a href=\"https:\/\/www.cell.com\/action\/doSearch?AllField=%22cell%20heterogeneity%22&amp;ISSN=0167-7799\">cell heterogeneity<\/a><\/li><li><a href=\"https:\/\/www.cell.com\/action\/doSearch?AllField=%22strain%20engineering%22&amp;ISSN=0167-7799\">strain engineering<\/a><\/li><li><a href=\"https:\/\/www.cell.com\/action\/doSearch?AllField=%22computational%20fluid%20dynamics%22&amp;ISSN=0167-7799\">computational fluid dynamics<\/a><\/li><li><a href=\"https:\/\/www.cell.com\/action\/doSearch?AllField=%22bioprocess%20engineering%22&amp;ISSN=0167-7799\">bioprocess engineering<\/a><\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Industrial cultivations suffer limitations in mixing and chemical feedstocks that cannot always match the laboratory-scale equivalent. As a result, normal laboratory-scale experiments do not capture relevant biological stresses, leading to underperformance at scale. Bacterial stress response can be studied from the point of view of industrial-scale stresses to better inform strain design. Strain design for [&#8230;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"none","nova_meta_subtitle":"Scale-down technology offers a relatively cost-effective way to mimic common industrial stresses on a laboratory scale","footnotes":""},"categories":[5572],"tags":[13383,5796,16588,12615],"supplier":[5112],"class_list":["post-128128","post","type-post","status-publish","format-standard","hentry","category-bio-based","tag-bacteria","tag-biotechnology","tag-cells","tag-microbes","supplier-elsevier"],"_links":{"self":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/128128","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/comments?post=128128"}],"version-history":[{"count":0,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/posts\/128128\/revisions"}],"wp:attachment":[{"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/media?parent=128128"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/categories?post=128128"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/tags?post=128128"},{"taxonomy":"supplier","embeddable":true,"href":"https:\/\/renewable-carbon.eu\/news\/wp-json\/wp\/v2\/supplier?post=128128"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}