{"id":101188,"date":"2021-11-29T07:38:00","date_gmt":"2021-11-29T06:38:00","guid":{"rendered":"https:\/\/renewable-carbon.eu\/news\/?p=101188"},"modified":"2021-11-24T14:01:24","modified_gmt":"2021-11-24T13:01:24","slug":"the-shikifactory100-project","status":"publish","type":"post","link":"https:\/\/renewable-carbon.eu\/news\/the-shikifactory100-project\/","title":{"rendered":"The SHIKIFACTORY100 – Modular cell factories for the production of 100 compounds from the shikimate pathway"},"content":{"rendered":"\n

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Figure 1: Metabolic pathway roadmap of eukaryotic cells. This overview image of metabolic pathways has been adapted by D. L. Nelson and M. M. Cox in ‘Principles of Biochemistry’ 4th Edition from the online KEGG (Kyoto Encyclopedia of Genes and Genomes) PATHWAY database (https:\/\/www.genome.jp\/pathway\/map01100).<\/figcaption><\/figure><\/div>\n\n\n\n

The SHIKIFACTORY100 project<\/a> aims towards the production of a universe of more than 100 high-added value compounds from the shikimate pathway, a hub in cell metabolism, through the development of an optimized shikimate chassis (based in 3 sub-hubs: Phe, Trp and Tyr) and the proposal and implementation of novel biosynthetic routes exploring enzyme promiscuity to introduce new pathways for the production of known and newly designed compounds.<\/strong><\/p>\n\n\n\n

Cell metabolism includes hundreds of crosslinked metabolic pathways, such as glycolysis, the Krebs cycle and the shikimate route. The latter allows organisms to synthesise aromatic amino acids, which play key roles in the metabolism of these organisms, including protein formation, regulation or catalysis. These molecules are not only important for metabolic purposes, but also industrially as they are used in the commercial preparation of food, pharma and chemical compounds. Nevertheless, current industrial production methods (chemical synthesis and extraction from biomass) present several challenges. This has created an interest in alternative production routes, such as microbial production, where living organisms such as bacteria and yeast are used for the production of the target compounds. A comprehensive article about the Shikimate Pathway is available here<\/a>.<\/p>\n\n\n\n

Project Video<\/h3>\n\n\n\n

The aim of the Shikifactory100 project is to use world-leading synthetic biology tools for the microbial production of 100 molecules derived from the shikimate pathway, with applications in food, pharma and cosmetics, using bacteria and yeasts as host organisms.

The partners have developed a short video explaining the scope of the project in more details. This video is accessible on YouTube via the following link and can also be accompanied by English, Spanish and Portuguese subtitles.<\/p>\n\n\n\n

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